IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/25

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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week 8</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Floor Two==
==Floor Two==
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The two ligation samples from the previous day were analysed by gel electrophoresis alongside controls (2b2L and 3b1L) and the samples 2b and 2c from the 22nd July.
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[[Image:BIORAD 2013-07-25 15hr 59min.JPG|thumb|Fig. Analysis of Ligation reactions by gel electrophoresis. Lane 1 and 2 contain samples 1 and 2, lanes 3 and 4 controls, lane 5 and 6 samples from 22nd July (2b and 2c)repectively]]
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Transformation reactions were prepared using SOC media for the ligation reactions, J04450 plasmid and sample 3b from 11/07 (J04450 with Nde1 site).
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The membrane was retrieved from the shaker and washed with TBST and TBS solutions respectively for ten minutes. It was then incubated in Anti His HRP conjugate solution. After further washing the membrane was imaged using chemiluminescent detection. This gave no visible result.
The membrane was retrieved from the shaker and washed with TBST and TBS solutions respectively for ten minutes. It was then incubated in Anti His HRP conjugate solution. After further washing the membrane was imaged using chemiluminescent detection. This gave no visible result.

Revision as of 09:48, 31 July 2013

Week 8 Main project page
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Floor Two

The two ligation samples from the previous day were analysed by gel electrophoresis alongside controls (2b2L and 3b1L) and the samples 2b and 2c from the 22nd July.

Fig. Analysis of Ligation reactions by gel electrophoresis. Lane 1 and 2 contain samples 1 and 2, lanes 3 and 4 controls, lane 5 and 6 samples from 22nd July (2b and 2c)repectively
Fig. Analysis of Ligation reactions by gel electrophoresis. Lane 1 and 2 contain samples 1 and 2, lanes 3 and 4 controls, lane 5 and 6 samples from 22nd July (2b and 2c)repectively

Transformation reactions were prepared using SOC media for the ligation reactions, J04450 plasmid and sample 3b from 11/07 (J04450 with Nde1 site).

The membrane was retrieved from the shaker and washed with TBST and TBS solutions respectively for ten minutes. It was then incubated in Anti His HRP conjugate solution. After further washing the membrane was imaged using chemiluminescent detection. This gave no visible result.



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