IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/25
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The membrane was retrieved from the shaker and washed with TBST and TBS solutions respectively for ten minutes. It was then incubated in Anti His HRP conjugate solution. After further washing the membrane was imaged using chemiluminescent detection. This gave no visible result.