IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/26

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(Floor Two)
(Floor Two)
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As the western blot from the previous day was unsuccessful, the protein analysis took a different path. The soluble protein fractions were purified using a His Mag solution to select the His Tagged AntA σ factor. These samples were ran on a 15% SDS PAGE along side the unpurified soluble fractions for 1 hour 25 minutes and stained with instant blue dye.  
As the western blot from the previous day was unsuccessful, the protein analysis took a different path. The soluble protein fractions were purified using a His Mag solution to select the His Tagged AntA σ factor. These samples were ran on a 15% SDS PAGE along side the unpurified soluble fractions for 1 hour 25 minutes and stained with instant blue dye.  
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[[Image:26 july.JPG|thumb|Fig. Analysis of colony PCR reactions. ]]
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[[Image:26 july.JPG|thumb|Fig. Analysis of colony PCR reactions. Lane 1 shows the negative control with no DNA, Lanes 2 and 3 are the positive controls with the original J04450 plasmid and J04450-Nde1 biobrick respectively. Lanes 4 and 5 are the J04450 colonies 1 and 2 respectively. Lanes 6 and 7 are the J04450- Nde1 biobrick colonies 1 and 2 respectively. Lanes 8 and 9 are the ligation reactions sample 1 colonies 1 and 2 respectively, while lanes 10 and 11 are are the ligation reactions sample 2 colonies 1 and 2 respectively]]
==Outreach==
==Outreach==

Revision as of 00:06, 6 August 2013

Week 8 Main project page
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Floor Two

The transformation reactions from the previous day were retrieved and colony Polymerase Chain Reactions (PCR) was preformed. The results were analysed by gel electrophoresis.

As the western blot from the previous day was unsuccessful, the protein analysis took a different path. The soluble protein fractions were purified using a His Mag solution to select the His Tagged AntA σ factor. These samples were ran on a 15% SDS PAGE along side the unpurified soluble fractions for 1 hour 25 minutes and stained with instant blue dye.

Fig. Analysis of colony PCR reactions. Lane 1 shows the negative control with no DNA, Lanes 2 and 3 are the positive controls with the original J04450 plasmid and J04450-Nde1 biobrick respectively. Lanes 4 and 5 are the J04450 colonies 1 and 2 respectively. Lanes 6 and 7 are the J04450- Nde1 biobrick colonies 1 and 2 respectively. Lanes 8 and 9 are the ligation reactions sample 1 colonies 1 and 2 respectively, while lanes 10 and 11 are are the ligation reactions sample 2 colonies 1 and 2 respectively
Fig. Analysis of colony PCR reactions. Lane 1 shows the negative control with no DNA, Lanes 2 and 3 are the positive controls with the original J04450 plasmid and J04450-Nde1 biobrick respectively. Lanes 4 and 5 are the J04450 colonies 1 and 2 respectively. Lanes 6 and 7 are the J04450- Nde1 biobrick colonies 1 and 2 respectively. Lanes 8 and 9 are the ligation reactions sample 1 colonies 1 and 2 respectively, while lanes 10 and 11 are are the ligation reactions sample 2 colonies 1 and 2 respectively

Outreach

Some members of the team attended an afternoon for summer students organised by the school of biological sciences at UEA. We spoke about iGEM and our project to other students and members of faculty.

Have been in contact with local Defra rep about obtaining a soil licence. Gathered all the correct forms and have handed them over to Matt Hutchings. I have had to put some sediment requests to the US on hold until the licence application is underway.

A date has been set for the Forum Event (28th August). Have been designing the advert + discussing what we will do for the event. Also we have identified local printing companies for posters/maps.

Also, I have arranged a meeting with Anne Osbourne at the JIC to open a discussion about how iGEM parts can be realistically integrated into real research projects. We are interested to see if our tool-based design could offer a realistic basis for real synthetic biology use in professional research institutions. Perhaps our project is more realistic than other iGEM projects. Will need to devise some questions for Anne before next Tuesday. Does this count as Outreach?? Still interesting if not.

Forum Advert Outreach Registry - contact other teams, write the message. Questions for Pro research labs




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