IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/29

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Restriction digest reactions were performed on the original J04450 original plasmid and the J04450-Nde1 bio brick using (i) EcoR1 and Pst1 and (ii) Nde1 and Xba1. After incubation samples were run on agarose gel. Transformation was done on the AntgP-Neo biobrick (ligated samples 2b and 2c). Also, colonies 1 and 2 (used in colony PCR) from samples 1 and 2 were inoculated and grown overnight.
Restriction digest reactions were performed on the original J04450 original plasmid and the J04450-Nde1 bio brick using (i) EcoR1 and Pst1 and (ii) Nde1 and Xba1. After incubation samples were run on agarose gel. Transformation was done on the AntgP-Neo biobrick (ligated samples 2b and 2c). Also, colonies 1 and 2 (used in colony PCR) from samples 1 and 2 were inoculated and grown overnight.
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[[Image:29 july.JPG|thumb|Fig. ]]
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[[Image:29 july.JPG|thumb| Restriction digest of J04450 original plasmid (lanes 1 and 3) and J04450-Nde1 biobrick (lanes 2 and 4) using i) EcoR1 and Pst1 and (ii) Nde1 and Xba1.]]

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Floor 2

Restriction digest reactions were performed on the original J04450 original plasmid and the J04450-Nde1 bio brick using (i) EcoR1 and Pst1 and (ii) Nde1 and Xba1. After incubation samples were run on agarose gel. Transformation was done on the AntgP-Neo biobrick (ligated samples 2b and 2c). Also, colonies 1 and 2 (used in colony PCR) from samples 1 and 2 were inoculated and grown overnight.

Restriction digest of J04450 original plasmid (lanes 1 and 3) and J04450-Nde1 biobrick (lanes 2 and 4) using i) EcoR1 and Pst1 and (ii) Nde1 and Xba1.
Restriction digest of J04450 original plasmid (lanes 1 and 3) and J04450-Nde1 biobrick (lanes 2 and 4) using i) EcoR1 and Pst1 and (ii) Nde1 and Xba1.



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