IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/02

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==Floor One==
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Spore stock preparation, filtering and concentrating (spinning down at 13 000 rpm for 1 min and pouring off supernatant) was continued.
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The conjugations were overlayed with 1 ml sterile water, 20 ul nalidixic acid and 100 ul kanamycin per plate. The plates were then incubated again overnight at 30 °C.
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N.B. These plates were accidentally left out at room temp. over the weekend so it is uncertain whether they will work - the E. coli may have had a chance to outcompete the Strep. (?).
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Streak-purified several contaminated single colony Streptomyces plates.
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==Floor Two==
==Floor Two==
The two cultures for the protein expression of σ AntA were retrieved from the incubator. 1.5ml was taken from each and centrifuged for five minutes. The supernatant was removed from each sample and the remaining pellets were stored in the freezer at -20°C.  
The two cultures for the protein expression of σ AntA were retrieved from the incubator. 1.5ml was taken from each and centrifuged for five minutes. The supernatant was removed from each sample and the remaining pellets were stored in the freezer at -20°C.  

Revision as of 13:05, 15 August 2013

Week Nine Main project page
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Floor One

Spore stock preparation, filtering and concentrating (spinning down at 13 000 rpm for 1 min and pouring off supernatant) was continued.

The conjugations were overlayed with 1 ml sterile water, 20 ul nalidixic acid and 100 ul kanamycin per plate. The plates were then incubated again overnight at 30 °C. N.B. These plates were accidentally left out at room temp. over the weekend so it is uncertain whether they will work - the E. coli may have had a chance to outcompete the Strep. (?).

Streak-purified several contaminated single colony Streptomyces plates.


Floor Two

The two cultures for the protein expression of σ AntA were retrieved from the incubator. 1.5ml was taken from each and centrifuged for five minutes. The supernatant was removed from each sample and the remaining pellets were stored in the freezer at -20°C.

Outreach

The Hotel for the European Jamboree in Lyon was booked.



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