IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/23

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(Autocreate 2013/08/23 Entry for IGEM:University_of_East_Anglia_(UEA),_Norwich,_UK/2009/Notebook/NRP-UEA-Norwich_iGEM)
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week Twelve</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
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==Floor Two==
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* Insert your content here.
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Our focus of the experiments was to prepare more DNA of the confirmed biobricks and to reclone our fourth brick. <br>
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To prepare biobrick J04450+neo; plasmids 3b and AntGpneo were cut with restriction enzymes NdeI and PstI. The samples were ran on a agarose gel and the large fragment of 3b and small fragment of AntGpneo were excised and purified seperately.<br>
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DNA from 3a and BBa_J04450 were transformed into α-select gold competent cells and spread on LB agar plates with added chlorophenicol antibiotics and incubated overnight at 37°C.  

Revision as of 08:15, 23 August 2013

Week Twelve Main project page
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Floor Two

Our focus of the experiments was to prepare more DNA of the confirmed biobricks and to reclone our fourth brick.
To prepare biobrick J04450+neo; plasmids 3b and AntGpneo were cut with restriction enzymes NdeI and PstI. The samples were ran on a agarose gel and the large fragment of 3b and small fragment of AntGpneo were excised and purified seperately.
DNA from 3a and BBa_J04450 were transformed into α-select gold competent cells and spread on LB agar plates with added chlorophenicol antibiotics and incubated overnight at 37°C.



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