IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/29

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(Autocreate 2013/08/29 Entry for IGEM:University_of_East_Anglia_(UEA),_Norwich,_UK/2009/Notebook/NRP-UEA-Norwich_iGEM)
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week Thirteen</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
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==Floor Two==
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* Insert your content here.
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As the agarose gel from the 22/08 was unsuccessful, the restriction digest of 3b and AntGpneo with PstI and NdeI was repeated. The BL21 cells that contain pETAntA and AntGpRFP plasmids were digested with XbaI. All samples were analysed by gel electrophoresis. <br>
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Two colonies from the plates containing samples 3a and Bba_J04450 were inoculated into 10ml cultures of LB and Chlorophenicol antibiotic. The four cultures (two colonies from each) were left to grow overnight at 37°C. 

Revision as of 08:17, 29 August 2013

Week Thirteen Main project page
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Floor Two

As the agarose gel from the 22/08 was unsuccessful, the restriction digest of 3b and AntGpneo with PstI and NdeI was repeated. The BL21 cells that contain pETAntA and AntGpRFP plasmids were digested with XbaI. All samples were analysed by gel electrophoresis.
Two colonies from the plates containing samples 3a and Bba_J04450 were inoculated into 10ml cultures of LB and Chlorophenicol antibiotic. The four cultures (two colonies from each) were left to grow overnight at 37°C.



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