IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/29

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Floor Two

As the agarose gel from the 22/08 was unsuccessful, the restriction digest of 3b and AntGpneo with PstI and NdeI was repeated. The BL21 cells that contain pETAntA and AntGpRFP plasmids were digested with XbaI. All samples were analysed by gel electrophoresis.
Two colonies from the plates containing samples 3a and Bba_J04450 were inoculated into 10ml cultures of LB and Chlorophenicol antibiotic. The four cultures (two colonies from each) were left to grow overnight at 37°C.



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