IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/29

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(Floor Two)
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==Floor Two==
==Floor Two==
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As the agarose gel from the 22/08 was unsuccessful, the restriction digest of 3b and AntGpneo with PstI and NdeI was repeated. The BL21 cells that contain pETAntA and AntGpRFP plasmids were digested with XbaI. All samples were analysed by gel electrophoresis. <br>
+
As the agarose gel from the 22/08 was unsuccessful, the restriction digest of 3b and AntGpneo with PstI and NdeI was repeated. The miniprep BL21 cells that contain pETAntA and AntGpRFP plasmids were digested with XbaI. All samples were analysed by gel electrophoresis. <br>
 +
The gel was successful and a large fragment from 3b and small fragment from AntGpneo were excised, purified and ligated overnight at 4°C.
Two colonies from the plates containing samples 3a and Bba_J04450 were inoculated into 10ml cultures of LB and Chlorophenicol antibiotic. The four cultures (two colonies from each) were left to grow overnight at 37°C.   
Two colonies from the plates containing samples 3a and Bba_J04450 were inoculated into 10ml cultures of LB and Chlorophenicol antibiotic. The four cultures (two colonies from each) were left to grow overnight at 37°C.   

Revision as of 08:11, 29 August 2013

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Floor Two

As the agarose gel from the 22/08 was unsuccessful, the restriction digest of 3b and AntGpneo with PstI and NdeI was repeated. The miniprep BL21 cells that contain pETAntA and AntGpRFP plasmids were digested with XbaI. All samples were analysed by gel electrophoresis.
The gel was successful and a large fragment from 3b and small fragment from AntGpneo were excised, purified and ligated overnight at 4°C. Two colonies from the plates containing samples 3a and Bba_J04450 were inoculated into 10ml cultures of LB and Chlorophenicol antibiotic. The four cultures (two colonies from each) were left to grow overnight at 37°C.



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