IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/09/04

From OpenWetWare

Jump to: navigation, search
(Autocreate 2013/09/04 Entry for IGEM:University_of_East_Anglia_(UEA),_Norwich,_UK/2009/Notebook/NRP-UEA-Norwich_iGEM)
Current revision (07:52, 25 September 2013) (view source)
(Entry title)
 
Line 5: Line 5:
|-
|-
| colspan="2"|
| colspan="2"|
-
==Entry title==
+
==Floor One==
-
* Insert your content here.
+
Performed chromosomal DNA preps for 12 of the O/N cultures that appeared to lack contaminants and had large areas of clearance in the Bioassays.<br>
 +
The ligated vector (pMS82 with GUS insert) was transformed into chemically-competent <i>E. coli</i> cells through heat shock. The cells were then plated onto LB-NaCl (with 200 μL Hygromycin per 200 ml LB). There were 2 plates with 100 μL and 800 μL volumes plated respectively per ligation reaction.

Current revision

iGEM Project name 1 Main project page
Previous entry      Next entry

Floor One

Performed chromosomal DNA preps for 12 of the O/N cultures that appeared to lack contaminants and had large areas of clearance in the Bioassays.
The ligated vector (pMS82 with GUS insert) was transformed into chemically-competent E. coli cells through heat shock. The cells were then plated onto LB-NaCl (with 200 μL Hygromycin per 200 ml LB). There were 2 plates with 100 μL and 800 μL volumes plated respectively per ligation reaction.



Personal tools