IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/09/11
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The three overnight cultures were retrieved from the incubator. Cultures three and four were used to inoculate four fresh 10ml LB liquids that contained Kanamycin and Chlorophenicol antibiotics. 100μL from each culture inoculated a fresh LB, resulting in two cultures per original culture. They were left in the shaking incubator at 37°C until OD600 reached 0.4-0.6. Half of the cultures were induced with IPTG and all the cultures were left shaking at 25°C for one hour. 100μL from each culture was spread on LB agar plates with Kanamycin and Chlorophenicol added and left to incubate overnight at 37°C.