IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/09/24
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Antibiotic overlays of the conjugation plates were performed - 1 ml of sterile water with 20 ul (25 mg/ml) Nalidixic acid and 25 ul (50 mg/ml) Apramycin was spread on top. The plates were incubated over the weekend at 30°C.