IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/08/04

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(Autocreate 2014/08/04 Entry for IGEM:University_of_East_Anglia_(UEA),_Norwich,_UK/2009/Notebook/NRP-UEA-Norwich_iGEM)
Current revision (10:48, 4 August 2014) (view source)
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==Entry title==
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==Week 8 Day 1==
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- Colony PCR of Agrobacterium plates containing constructs 2,3,4,10,11 which had been grown over the weekend at 28 °C.  
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- Colony PCR products run on a 1% agarose TAE gel with a DNA positive control.
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- Picked single colonies of Level 1 constructs: 1, 5, 9, 15, 16, 17, 18, 19. Colony PCR of these level 1 constructs.
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- 2 Agrobacterium colonies selected from each plate to be grown in 5 ml of LB, 100 µl Ampicillin, 200 µl Gentamycin and 200 µl Rifampicin O/N at 28 °C with shaking.
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Human Practices
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- 93 agar plates autoclaved and poured for Human Practices school events in September, kept in cold room.
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Week 8 Day 1

- Colony PCR of Agrobacterium plates containing constructs 2,3,4,10,11 which had been grown over the weekend at 28 °C.

- Colony PCR products run on a 1% agarose TAE gel with a DNA positive control.

- Picked single colonies of Level 1 constructs: 1, 5, 9, 15, 16, 17, 18, 19. Colony PCR of these level 1 constructs.

- 2 Agrobacterium colonies selected from each plate to be grown in 5 ml of LB, 100 µl Ampicillin, 200 µl Gentamycin and 200 µl Rifampicin O/N at 28 °C with shaking.


Human Practices


- 93 agar plates autoclaved and poured for Human Practices school events in September, kept in cold room.




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