IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/06/29
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6/29/105:30PM Digestion of backbones Samples: pSB1A2, pSB1C3, pSB1T3, pSB2K3 (BBa_E0033), pSB1AC3, pSB1AK3, pSB1AT3
Incubate at 37 º C, 15 min Inactivate at 80 º C, 20 min PCR cleanup add 2 ul SAP buffer, 1 ul SAP Incubate 37 º C, 10 min Incubate 65 º C, 15 min Gel 175 V, 1% TAE
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