IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/07/28
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2/28/10Got colonies on both LamB plates! 10:00 AM PCR
Per Reaction: 18.6 μL mastermix, 1 μL template,.2 μL F primer, .2 μL R primer. Primers: 20 micromolar VF2 VR Samples: ArsR SDM1 ArsR SDM2 A1 A2 B1 B2 L1 RFP 1 L1 RFP 2 L3 GFP 1 L3 GFP 2 GolS Program:
10:50 AM PCR
Per reaction: 19.2 μL mastermix, .4 μL F primer, .4 μL R primer. Primers: 10 micromolar ArsBF2 ArsBR2 samples: ArsB 48°C ArsB 51°C ArsB 54°C ArsB 57°C ArsB 60°C ArsB 63°C ArsB 66°C Negative control (no primers) Program:
1:20 PM Gel Top
Bottom:
<html> <table border="1" cellpadding="7"> <tr> <td> Lane </td> <td> Sample </td> <td> Amount </td> </td> <tr> <td> 1 </td> <td> 1 Kb Ladder </td> <td>5 μL sample, 1 μL dye </td> </td> <tr> <td> 2</td> <td> ArsB 66°C</td> <td>5 μL sample, 1 μL dye </td> </td> <tr> <td> 3</td> <td> Tom1 </td> <td> 15 μL sample, 3 μL dye</td> </td> <tr> <td> 4</td> <td> Tom2</td> <td>15 μL sample, 3 μL dye </td> </td> <tr> <td> 5</td> <td> Tom3</td> <td> 15 μL sample, 3 μL dye</td> </td> <tr> <td>6 </td> <td> Tom4</td> <td> 15 μL sample, 3 μL dye</td> </tr> </table> </html> |