IGEM:VGEM/2007/Notebook/2007-6-25: Difference between revisions
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(New page: ==June 25, 2007== *Kevin's paper presentation *Observed Z competent cells grown on Friday **Found little RFP *Made new broth from Z competent cells **Added 5 mL broth **Added scraping of c...) |
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==June 25, 2007== | ==June 25, 2007== | ||
*Kevin's paper presentation | *Kevin's paper presentation | ||
*Observed Z competent cells grown on Friday | *Observed Z-competent cells grown on Friday | ||
**Found little RFP | **Found little RFP | ||
*Made new broth from Z competent cells | *Made new broth from Z-competent cells | ||
**Added 5 mL broth | **Added 5 mL broth | ||
**Added scraping of cells from sterilized innoculating loop | **Added scraping of cells from sterilized innoculating loop | ||
**Vortexed for 6-10 seconds | **Vortexed for 6-10 seconds | ||
**Incubated in shaker @ ~36.5 +/- 0.5 degrees C | **Incubated in shaker @ ~36.5 +/- 0.5 degrees C | ||
*Incubated Z-competent cells from Friday in shaker @ ~36.5 +/- 0.5 degrees C again | |||
*Began working on presentation to Medical School | *Began working on presentation to Medical School | ||
Revision as of 07:25, 25 June 2007
June 25, 2007
- Kevin's paper presentation
- Observed Z-competent cells grown on Friday
- Found little RFP
- Made new broth from Z-competent cells
- Added 5 mL broth
- Added scraping of cells from sterilized innoculating loop
- Vortexed for 6-10 seconds
- Incubated in shaker @ ~36.5 +/- 0.5 degrees C
- Incubated Z-competent cells from Friday in shaker @ ~36.5 +/- 0.5 degrees C again
- Began working on presentation to Medical School
