IGEM:VGEM/2007/Notebook/2007-6-25: Difference between revisions
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==June 25, 2007== | ==June 25, 2007== | ||
===AM=== | |||
*Kevin's paper presentation | *Kevin's paper presentation | ||
*Observed Z-competent cells grown on Friday | *Observed Z-competent cells grown on Friday | ||
| Line 10: | Line 11: | ||
*Incubated Z-competent cells from Friday in shaker @ ~36.5 +/- 0.5 degrees C again | *Incubated Z-competent cells from Friday in shaker @ ~36.5 +/- 0.5 degrees C again | ||
*Began working on presentation to Medical School | *Began working on presentation to Medical School | ||
===PM=== | |||
*Discussed [http://www.nature.com/nbt/journal/v21/n7/pdf/nbt833.pdf ''Engineering a Mevalonate Pathway in Escherichia coli for Production of Terpenoids''] | |||
*Researched project ideas | |||
Latest revision as of 12:24, 25 June 2007
June 25, 2007
AM
- Kevin's paper presentation
- Observed Z-competent cells grown on Friday
- Found little RFP
- Made new broth from Z-competent cells
- Added 5 mL broth
- Added scraping of cells from sterilized innoculating loop
- Vortexed for 6-10 seconds
- Incubated in shaker @ ~36.5 +/- 0.5 degrees C
- Incubated Z-competent cells from Friday in shaker @ ~36.5 +/- 0.5 degrees C again
- Began working on presentation to Medical School
PM
- Discussed Engineering a Mevalonate Pathway in Escherichia coli for Production of Terpenoids
- Researched project ideas
