IGEM:VGEM/2007/Notebook/2007-6-6: Difference between revisions
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(First runthrough with GFP biobrick) |
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*Biobrick Construction Tutorial | *Biobrick Construction Tutorial | ||
**Transformation of ''E. coli'' with BBa_I13522 from Plate 3, Well 13C. | |||
**Cell type: subcloning efficiency DH5alpha<br/ > | |||
*What was done: | |||
**Bacteria were transformed to express GFP (with the aforementioned Biobrick) | |||
**20 uL and 200 uL of transformed bacteria were plated and incubated overnight. | |||
**20 uL and 200 uL of nontransformed bacteria were plated and incubated overnight as a control. | |||
**Total of 4 plates | |||
**~5 uL of suspended Biobrick leftover | |||
Revision as of 09:42, 7 June 2007
- Biobrick Construction Tutorial
- Transformation of E. coli with BBa_I13522 from Plate 3, Well 13C.
- Cell type: subcloning efficiency DH5alpha
- What was done:
- Bacteria were transformed to express GFP (with the aforementioned Biobrick)
- 20 uL and 200 uL of transformed bacteria were plated and incubated overnight.
- 20 uL and 200 uL of nontransformed bacteria were plated and incubated overnight as a control.
- Total of 4 plates
- ~5 uL of suspended Biobrick leftover
