- Making Glycerol Stock of GFP transformed cells
- We verified the expression of GFP in our cells. It was a great moment in the dark Wilsdorf closet.
- We made a 50:50 cell to glycerol solution and stored it in the minus 20C.
- We plated another 200uL of cells onto Amp+ plates. We hope to have more colonies on this plate.
ERuhi 11:58, 8 June 2007 (EDT)
Protocol: Making glycerol stock for long term storage:
1. Add 1 ml of 40% glycerol in H2O to a cryogenic vial. 2. Add 1 ml sample from the culture of bacteria to be stored. 3. Gently vortex the cryogenic vial to ensure the culture and glycerol is well-mixed. 1. Alternatively, pipet to mix. 4. On the side of the vial list all relevant information - part, vector, strain, date, researcher, etc. 5. Store in a freezer box in a -80C freezer. Remember to record where the vial is stored for fast retrieval later.
Note: We were sucessful in transforming our cells with the biobrick ERuhi 21:41, 8 June 2007 (EDT)