IGEM:VGEM/2007/Notebook/2007-8-1: Difference between revisions
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'''Expect synthetic DNA from GENEART for melanin project''' | '''Expect synthetic DNA from GENEART for melanin project''' | ||
*Making Minimal Medium: | |||
**Add 495mL of distilled water to 3.0g Na2HPO4, 1.5g KH2PO4, 0.25g NaCl, 0.5g NH4Cl | |||
**Repeat above step in another flask with the addition of 7g of agar | |||
**Adjust pH to 7.4 | |||
**Autoclave at 121oC | |||
**Add 3.02g MgSO4 powder to 25mL of water to make 1M solution | |||
**Add 8g glucose to 32mL water | |||
**Autoclave | |||
**Add 1.0mL 1M MgSO4 solution to broth and to agar solutions | |||
**Add 5.0mL of 20% glucose solution to broth and agar solutions | |||
**After cooling, pour agar solution into plates | |||
Extracting and plating K12 Cells: | |||
**Extract K12 cells from glass vial by heating the tip over a bunson burner flame then cold shocking it | |||
** | |||
**Incubate tube and plates at 37oC for 24hr | |||
Latest revision as of 11:43, 1 August 2007
Expect synthetic DNA from GENEART for melanin project
- Making Minimal Medium:
- Add 495mL of distilled water to 3.0g Na2HPO4, 1.5g KH2PO4, 0.25g NaCl, 0.5g NH4Cl
- Repeat above step in another flask with the addition of 7g of agar
- Adjust pH to 7.4
- Autoclave at 121oC
- Add 3.02g MgSO4 powder to 25mL of water to make 1M solution
- Add 8g glucose to 32mL water
- Autoclave
- Add 1.0mL 1M MgSO4 solution to broth and to agar solutions
- Add 5.0mL of 20% glucose solution to broth and agar solutions
- After cooling, pour agar solution into plates
Extracting and plating K12 Cells:
- Extract K12 cells from glass vial by heating the tip over a bunson burner flame then cold shocking it
- Incubate tube and plates at 37oC for 24hr
