IGEM:VGEM/2007/Notebook/2007-8-3: Difference between revisions
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*Made stock solution of 2nd generation K12 cells | |||
*Made LB control plates | |||
*Made stock solutions of minimal media and LB | |||
*Grow up K12 cells from 2nd dilution | |||
*Began butanol tolerance experiment: | |||
**Made LB broth solutions containing 100uL of cells and the following butanol concentrations: | |||
**0, 0.4, 0.8, 1.2, 1.6, 2.0, 2.4, 2.8, 3.2% | |||
**Cells went into incubator/shaker at 2PM | |||
Latest revision as of 11:07, 3 August 2007
- Made stock solution of 2nd generation K12 cells
- Made LB control plates
- Made stock solutions of minimal media and LB
- Grow up K12 cells from 2nd dilution
- Began butanol tolerance experiment:
- Made LB broth solutions containing 100uL of cells and the following butanol concentrations:
- 0, 0.4, 0.8, 1.2, 1.6, 2.0, 2.4, 2.8, 3.2%
- Cells went into incubator/shaker at 2PM
