IGEM:VGEM/2007/Projects

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This system incorporates the idea of the repressilator on a larger scale, using three distinct cell types that are chemically isolated from each other (i.e., not sharing medium) and are each equipped with genes that enable bioluminescence and photosensing.  Cell type 1 is bioluminescent at a particular wavelength (e.g., blue).  Cell type 2 produces yellow bioluminescence unless it perceives blue light.  Cell type 3 produces bioluminescence at yet another wavelength (e.g., green) unless it senses yellow light.  Green light represses cell type 1 blue bioluminescence.  Thus, engineered cell-cell communication with light is possible, creating a "wireless" repressilator system.
This system incorporates the idea of the repressilator on a larger scale, using three distinct cell types that are chemically isolated from each other (i.e., not sharing medium) and are each equipped with genes that enable bioluminescence and photosensing.  Cell type 1 is bioluminescent at a particular wavelength (e.g., blue).  Cell type 2 produces yellow bioluminescence unless it perceives blue light.  Cell type 3 produces bioluminescence at yet another wavelength (e.g., green) unless it senses yellow light.  Green light represses cell type 1 blue bioluminescence.  Thus, engineered cell-cell communication with light is possible, creating a "wireless" repressilator system.
====References====
====References====
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Add
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*[http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=7832590&ordinalpos=119&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum Photobiology of bacteria]
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*Differential activation of E. coli chemoreceptors by blue-light stimuli
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===Directed Angiogenesis===
===Directed Angiogenesis===
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Coming up with alternative fuels is a real-world problem.  We're interested in using cheap, renewable feedstock to power efficient biofuel production.  More to come!
Coming up with alternative fuels is a real-world problem.  We're interested in using cheap, renewable feedstock to power efficient biofuel production.  More to come!
====References====
====References====
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Add
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*[http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=17372221&ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum Proteorhodopsin photosystem gene expression enables photophosphorylation in a heterologous host] by DeLong ''et al''
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*[http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=17277079&ordinalpos=2&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum Light-powering E. coli with proteorhodopsin]
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*[http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=16008504&ordinalpos=7&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSumNew  Insights into metabolic properties of marine bacteria encoding proteorhodopsins]
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*[http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=16707677&ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum  Complete cellulase system in the marine bacterium ''Saccharophagus degradans'' strain 2-40T] by Weiner ''et al''
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*[http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=16014479&ordinalpos=4&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum Saccharophagus degradans, a versatile marine degrader of complex polysaccharides]
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*[http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=17311090&ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum Plant Carbohydrate Scavenging through TonB-Dependent Receptors: A Feature Shared by Phytopathogenic and Aquatic Bacteria]
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*[http://aem.asm.org/cgi/content/abstract/68/1/53 Improvement of Cellulolytic Properties of Clostridium cellulolyticum by Metabolic Engineering]
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*[http://www3.interscience.wiley.com/cgi-bin/abstract/109792702/ABSTRACT?CRETRY=1&SRETRY=0 Butanol fermentation research: upstream and downstream manipulations]
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*[http://www3.interscience.wiley.com/cgi-bin/abstract/114109891/ABSTRACT Butanol production from agricultural residues: Impact of degradation products on ''Clostridium beijerinckii'' growth and butanol fermentation]
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*[http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6VRV-4NK4739-1&_user=709071&_coverDate=06%2F30%2F2007&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000039638&_version=1&_urlVersion=0&_userid=709071&md5=cd81c68d18f23cafdc69fa0d8a90c1b6 Bioproduction of butanol from biomass: from genes to bioreactors]
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*[http://aem.asm.org/cgi/content/full/73/9/3061?view=long&pmid=17337545 Dynamics of Genomic-Library Enrichment and Identification of Solvent Tolerance Genes for Clostridium acetobutylicum]
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*[http://pubs3.acs.org/acs/journals/doilookup?in_doi=10.1021/bp050360w Butanol Production from Corn Fiber Xylan Using ''Clostridium acetobutylicum'']
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*[http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=16685494&ordinalpos=10&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum Bacterial acetone and butanol production by industrial fermentation in the Soviet Union: use of hydrolyzed agricultural waste for biorefinery]
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*[http://aem.asm.org/cgi/content/abstract/45/3/966 Butanol Production by a Butanol-Tolerant Strain of ''Clostridium acetobutylicum'' in Extruded Corn Broth]
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*[http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=17289987&ordinalpos=4&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum Challenges in engineering microbes for biofuel production] by Stephanopoulos
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*[http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=16270654&ordinalpos=9&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum Metabolic engineering] by Stephanopoulos
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*[http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=16041571&ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVAbstractPlus Global physiological understanding and metabolic engineering of microorganisms based on omics studies] by Park ''et al''

Revision as of 13:20, 1 August 2007

Contents

Projects

The following project descriptions are of systems that we will attempt to construct this summer. Additional project ideas may be added in the near future.


Bacterial Melanogenesis



This project is an extension of work that was done at Biosource Genetics Corporation in 1990. What we would like to do is program bacteria to produce melanin in response to a red light stimulus. This inducible melanin production has various applications including making melanin for monitoring cellular processes. In this way, melanin would serve as a biomarker or indicator similar to the GFP but would be easily visible to the naked eye.

References


Ethylene Biosensor

NOTE: This project has been put on the back burner.


During one of our brainstorming sessions we were discussing possible biosensors and came up with a fairly practical project idea: an ethylene biosensor. Why sense ethylene? Mature fruit produce and release ethylene as they ripen. Measuring the concentration of gaseous ethylene on or near the surface of the ripening fruit would allow for the indirect measurement of its degree of ripeness.

References


Synthetic Biological Clock

NOTE: This project has been put on the back burner.


The synthetic biological clock was one of our earliest project ideas and involves the coupling of Elowitz and Leibler's repressilator system to some actuator such as fluorescence or aroma generation. We would like to link MIT's 2006 iGEM project to the repressilator and create an aroma therapy clock in addition to linking green, yellow and red fluorescent proteins to the repressilator to make a molecular traffic light. Future applications of controlled synthetic oscillatory systems include internal, autonomous drug delivery technology.

References


Cellular Photosignalling


This system incorporates the idea of the repressilator on a larger scale, using three distinct cell types that are chemically isolated from each other (i.e., not sharing medium) and are each equipped with genes that enable bioluminescence and photosensing. Cell type 1 is bioluminescent at a particular wavelength (e.g., blue). Cell type 2 produces yellow bioluminescence unless it perceives blue light. Cell type 3 produces bioluminescence at yet another wavelength (e.g., green) unless it senses yellow light. Green light represses cell type 1 blue bioluminescence. Thus, engineered cell-cell communication with light is possible, creating a "wireless" repressilator system.

References


Directed Angiogenesis


Natural phototaxis systems can be exploited to direct engineered bacteria to target areas within the body. These living machines are programmed so that once they have arrived, they produce and secrete their payload (e.g., VEGF).

References

Add


Biofuel Manufacturing


Coming up with alternative fuels is a real-world problem. We're interested in using cheap, renewable feedstock to power efficient biofuel production. More to come!

References

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