This project is an extension of work that was done at Biosource Genetics Corporation in 1990. What we would like to do is program bacteria to produce melanin in response to a red light stimulus. This inducible melanin production has various applications including making melanin for monitoring cellular processes. In this way, melanin would serve as a biomarker or indicator similar to the GFP but would be easily visible to the naked eye.
- Melanin production in Escherichia coli from a cloned tyrosinase gene by Della-Cioppa G, Garger SJ, Sverlow GG, Turpen TH, Grill LK.
During one of our brainstorming sessions we were discussing possible biosensors and came up with a fairly practical project idea: an ethylene biosensor. Why sense ethylene? Mature fruit produce and release ethylene as they ripen. Measuring the concentration of gaseous ethylene on or near the surface of the ripening fruit would allow for the indirect measurement of its degree of ripeness.