IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/06/04: Difference between revisions

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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;">Week 2</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
==(6/4 – 6/9)==
* Insert your content here.
Attempted genome isolation. We also familiarized ourselves with the basics of
the Nanodrop machine. We would check by quantifying the phage genome with
Nanodrop. However we got a flat curve indicating that it did not work.
<br>
Sadly, chemical transformation did not work on Bordatella Bronchiseptica.
<br>
Performed gel extraction with gel a gel extraction kit along with primers
Started 2 300mL cultures of Bordatella Bronchiseptica, added 20 uL phage for
amplification and took time points. (morning and evening for 48 hours). We
quantified with qPCR. Ran it twice (without diluting phage from each time point).
PCR did not work on Bordatella plate
<br>
Tried to use a plaque assay on Bronchiseptica with differing amounts of phage to
quantify the different time points.
<br>
Attempted freeze/thaw transformation in Bronchiseptica, which did not work.




Revision as of 17:13, 3 October 2012

Week 2 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

(6/4 – 6/9)

Attempted genome isolation. We also familiarized ourselves with the basics of the Nanodrop machine. We would check by quantifying the phage genome with Nanodrop. However we got a flat curve indicating that it did not work.
Sadly, chemical transformation did not work on Bordatella Bronchiseptica.
Performed gel extraction with gel a gel extraction kit along with primers Started 2 300mL cultures of Bordatella Bronchiseptica, added 20 uL phage for amplification and took time points. (morning and evening for 48 hours). We quantified with qPCR. Ran it twice (without diluting phage from each time point). PCR did not work on Bordatella plate
Tried to use a plaque assay on Bronchiseptica with differing amounts of phage to quantify the different time points.
Attempted freeze/thaw transformation in Bronchiseptica, which did not work.



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