IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/06/04: Difference between revisions
(Autocreate 2012/06/04 Entry for IGEM:Virginia/2012/Notebook/Genetically_engineered_bacteriophage_for_diagnosis_of_whooping_cough) |
(fix raw html notebook nav) |
||
(One intermediate revision by one other user not shown) | |||
Line 1: | Line 1: | ||
{|{{table}} width="800" | {|{{table}} width="800" | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;">Week 2</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
== | ==(6/4 – 6/9)== | ||
Attempted genome isolation. We also familiarized ourselves with the basics of | |||
the Nanodrop machine. We would check by quantifying the phage genome with | |||
Nanodrop. However we got a flat curve indicating that it did not work. | |||
<br> | |||
Sadly, chemical transformation did not work on Bordatella Bronchiseptica. | |||
<br> | |||
Performed gel extraction with gel a gel extraction kit along with primers | |||
Started 2 300mL cultures of Bordatella Bronchiseptica, added 20 uL phage for | |||
amplification and took time points. (morning and evening for 48 hours). We | |||
quantified with qPCR. Ran it twice (without diluting phage from each time point). | |||
PCR did not work on Bordatella plate | |||
<br> | |||
Tried to use a plaque assay on Bronchiseptica with differing amounts of phage to | |||
quantify the different time points. | |||
<br> | |||
Attempted freeze/thaw transformation in Bronchiseptica, which did not work. | |||
Latest revision as of 22:05, 26 September 2017
Week 2 | Main project page Previous entry Next entry |
(6/4 – 6/9)Attempted genome isolation. We also familiarized ourselves with the basics of
the Nanodrop machine. We would check by quantifying the phage genome with
Nanodrop. However we got a flat curve indicating that it did not work.
|