IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/06/11: Difference between revisions
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week 3</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==(6/11 – 6-16)== | ||
Pipetted RB50 with plasmid and RB50 without plasmid on kanamycin plates and left | |||
them for incubation. | |||
<br> | |||
• We set up PCR for luciferase. | |||
<br> | |||
• Tried chemical transformation on RB50 with plasmid and without plasmid as well | |||
as DH5-alpha (as a positive control). We planned to transform with kanamycin | |||
resistance plasmid. | |||
<br> | |||
• Imaged gel from PCR of Luciferase/Kanamycin plasmids. It was successful. | |||
<br> | |||
• Used Qiagen PCR purification kit to clean up amplicons. | |||
<br> | |||
• Results of transformation: DH5-alpha grew. RB50 with and without plasmid, | |||
however, did not grow. | |||
<br> | |||
• Looking for ways to electroporate the DNA into the cells. | |||
Latest revision as of 22:05, 26 September 2017
Week 3 | Main project page Previous entry Next entry |
(6/11 – 6-16)Pipetted RB50 with plasmid and RB50 without plasmid on kanamycin plates and left
them for incubation.
|