IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/06/11

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(Autocreate 2012/06/11 Entry for IGEM:Virginia/2012/Notebook/Genetically_engineered_bacteriophage_for_diagnosis_of_whooping_cough)
Current revision (20:14, 3 October 2012) (view source)
 
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week 3</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
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==(6/11 – 6-16)==
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* Insert your content here.
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Pipetted RB50 with plasmid and RB50 without plasmid on kanamycin plates and left
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them for incubation.
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<br>
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• We set up PCR for luciferase.
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<br>
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• Tried chemical transformation on RB50 with plasmid and without plasmid as well
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as DH5-alpha (as a positive control). We planned to transform with kanamycin
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resistance plasmid.
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<br>
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• Imaged gel from PCR of Luciferase/Kanamycin plasmids. It was successful.
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<br>
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• Used Qiagen PCR purification kit to clean up amplicons.
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<br>
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• Results of transformation: DH5-alpha grew. RB50 with and without plasmid,
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however, did not grow.
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<br>
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• Looking for ways to electroporate the DNA into the cells.

Current revision

Week 3 Main project page
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(6/11 – 6-16)

Pipetted RB50 with plasmid and RB50 without plasmid on kanamycin plates and left them for incubation.
• We set up PCR for luciferase.
• Tried chemical transformation on RB50 with plasmid and without plasmid as well as DH5-alpha (as a positive control). We planned to transform with kanamycin resistance plasmid.
• Imaged gel from PCR of Luciferase/Kanamycin plasmids. It was successful.
• Used Qiagen PCR purification kit to clean up amplicons.
• Results of transformation: DH5-alpha grew. RB50 with and without plasmid, however, did not grow.
• Looking for ways to electroporate the DNA into the cells.



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