IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/06/11: Difference between revisions

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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week 3</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
==(6/11 – 6-16)==
* Insert your content here.
Pipetted RB50 with plasmid and RB50 without plasmid on kanamycin plates and left
them for incubation.
<br>
• We set up PCR for luciferase.
<br>
• Tried chemical transformation on RB50 with plasmid and without plasmid as well
as DH5-alpha (as a positive control). We planned to transform with kanamycin
resistance plasmid.
<br>
• Imaged gel from PCR of Luciferase/Kanamycin plasmids. It was successful.
<br>
• Used Qiagen PCR purification kit to clean up amplicons.
<br>
• Results of transformation: DH5-alpha grew. RB50 with and without plasmid,
however, did not grow.
<br>
• Looking for ways to electroporate the DNA into the cells.




Revision as of 17:14, 3 October 2012

Week 3 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

(6/11 – 6-16)

Pipetted RB50 with plasmid and RB50 without plasmid on kanamycin plates and left them for incubation.
• We set up PCR for luciferase.
• Tried chemical transformation on RB50 with plasmid and without plasmid as well as DH5-alpha (as a positive control). We planned to transform with kanamycin resistance plasmid.
• Imaged gel from PCR of Luciferase/Kanamycin plasmids. It was successful.
• Used Qiagen PCR purification kit to clean up amplicons.
• Results of transformation: DH5-alpha grew. RB50 with and without plasmid, however, did not grow.
• Looking for ways to electroporate the DNA into the cells.



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