IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/06/11

From OpenWetWare
Jump to navigationJump to search
The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.
Week 3 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

(6/11 – 6-16)

Pipetted RB50 with plasmid and RB50 without plasmid on kanamycin plates and left them for incubation.
• We set up PCR for luciferase.
• Tried chemical transformation on RB50 with plasmid and without plasmid as well as DH5-alpha (as a positive control). We planned to transform with kanamycin resistance plasmid.
• Imaged gel from PCR of Luciferase/Kanamycin plasmids. It was successful.
• Used Qiagen PCR purification kit to clean up amplicons.
• Results of transformation: DH5-alpha grew. RB50 with and without plasmid, however, did not grow.
• Looking for ways to electroporate the DNA into the cells.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:Virginia/2012/Notebook/Genetically_engineered_bacteriophage_for_diagnosis_of_whooping_cough/2012/06/11&oldid=632260"