IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/06/17: Difference between revisions

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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week 4</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
==(6/17 – 6/23)==
* Insert your content here.
Attempted electrotransformation of Bordatella to electroporate DNA into the cells.
This did not work. We found that RB50 and RB53 are not electrotransformable
<br>
Used Qiagen Viral RNA kit to purify phage genome and then did nanodrop
successfully. Also performed gel electrophoresis to visualize size and quantity of
DNA.
<br>
Performed PCR on isolated phage genome BPP
<br>
Gel electrophoresis image confirmed that the start and end of the BPP phage genome
were what we thought they were.
<br>
The 260/280 ratio for nanodrop of the genomic phage DNA isolation was off. It
needed to be closer to 1.8.
<br>
We attempted isolation of the hCG plasmid from DH5-alpha using Qiagen prep spin
miniprep kit.




Revision as of 17:16, 3 October 2012

Week 4 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

(6/17 – 6/23)

Attempted electrotransformation of Bordatella to electroporate DNA into the cells. This did not work. We found that RB50 and RB53 are not electrotransformable
Used Qiagen Viral RNA kit to purify phage genome and then did nanodrop successfully. Also performed gel electrophoresis to visualize size and quantity of DNA.
Performed PCR on isolated phage genome BPP
Gel electrophoresis image confirmed that the start and end of the BPP phage genome were what we thought they were.
The 260/280 ratio for nanodrop of the genomic phage DNA isolation was off. It needed to be closer to 1.8.
We attempted isolation of the hCG plasmid from DH5-alpha using Qiagen prep spin miniprep kit.



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