Jacqueline K. Grimm at 00:16, 4 October 2012

2012-10-04T00:16:05Z

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-	\|style="background-color: #EEE"\|[[Image:igem-logo-150px.png\|150px]]<span style="font-size:22px;"> ~~iGEM Project name 1~~</span>	+	\|style="background-color: #EEE"\|[[Image:igem-logo-150px.png\|150px]]<span style="font-size:22px;"> Week 4</span>
	\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}		\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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-	==~~Entry title~~==	+	==(6/17 – 6/23)==
-	* Insert your content here.	+	Attempted electrotransformation of Bordatella to electroporate DNA into the cells.
		+	This did not work. We found that RB50 and RB53 are not electrotransformable
		+	<br>
		+	Used Qiagen Viral RNA kit to purify phage genome and then did nanodrop
		+	successfully. Also performed gel electrophoresis to visualize size and quantity of
		+	DNA.
		+	<br>
		+	Performed PCR on isolated phage genome BPP
		+	<br>
		+	Gel electrophoresis image confirmed that the start and end of the BPP phage genome
		+	were what we thought they were.
		+	<br>
		+	The 260/280 ratio for nanodrop of the genomic phage DNA isolation was off. It
		+	needed to be closer to 1.8.
		+	<br>
		+	We attempted isolation of the hCG plasmid from DH5-alpha using Qiagen prep spin
		+	miniprep kit.

Jacqueline K. Grimm: Autocreate 2012/06/17 Entry for IGEM:Virginia/2012/Notebook/Genetically_engineered_bacteriophage_for_diagnosis_of_whooping_cough

2012-10-04T00:15:08Z

Autocreate 2012/06/17 Entry for IGEM:Virginia/2012/Notebook/Genetically_engineered_bacteriophage_for_diagnosis_of_whooping_cough

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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
* Insert your content here.


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__NOTOC__

IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/06/17 - Revision history

Jacqueline K. Grimm at 00:16, 4 October 2012

Jacqueline K. Grimm: Autocreate 2012/06/17 Entry for IGEM:Virginia/2012/Notebook/Genetically_engineered_bacteriophage_for_diagnosis_of_whooping_cough