IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/06/24: Difference between revisions
(Autocreate 2012/06/24 Entry for IGEM:Virginia/2012/Notebook/Genetically_engineered_bacteriophage_for_diagnosis_of_whooping_cough) |
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==Entry title== | ==Entry title== | ||
Performed Gibson Assembly on Luc genome and hCG genome. We then performed | |||
digest on the Gibson Assembly products. The results were hard to decipher. | |||
<br> | |||
We tried various lysis techniques such as: BPER buffer, chloroform, and RIPA buffer. | |||
<br> | |||
Bought EPT and Clearblue pregnancy test kits. Their purpose was to detect for the | |||
presence of hCG. Unfortunately we got all negative results. We tried with E. coli first, | |||
but still got negative results meaning that it did not take up the hCG gene. | |||
<br> | |||
Tried additional sonication method for lysis. | |||
<br> | |||
Digest of phage genome and vector digest both worked. During PCR purification, | |||
the products were resuspended in too large a volume, thereby making them dilute. | |||
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The next step was to either concentrate them or run a larger volume of digested hCG | |||
plasmid in order to detect via traniluminator. | |||
Revision as of 17:16, 3 October 2012
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Entry titlePerformed Gibson Assembly on Luc genome and hCG genome. We then performed
digest on the Gibson Assembly products. The results were hard to decipher.
|