IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/07/01
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< IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough | 2012 | 07(Difference between revisions)
(Autocreate 2012/07/01 Entry for IGEM:Virginia/2012/Notebook/Genetically_engineered_bacteriophage_for_diagnosis_of_whooping_cough) |
Current revision (20:18, 3 October 2012) (view source) |
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| - | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> | + | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week 6</span> |
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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| colspan="2"| | | colspan="2"| | ||
| - | == | + | ==(7/1 – 7/7)== |
| - | + | Attempted to find antibiotics to which RB53 was resistant to. | |
| + | <br> | ||
| + | Performed plasmid miniprep on tube # 3,5,7,10 and then did nanodrop. The | ||
| + | nanodrop values were very low, so we added a large volume of template DNA to the | ||
| + | PCR reaction. | ||
| + | <br> | ||
| + | Did PCR clean up on hCG digest. | ||
| + | <br> | ||
| + | Performed nanodrop on cleaned up, CAIP-treated, and digested PET vector. The | ||
| + | nanodrop, however, did not show any DNA present. | ||
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Week 6
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(7/1 – 7/7)Attempted to find antibiotics to which RB53 was resistant to.
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