IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/08/05: Difference between revisions
(Autocreate 2012/08/05 Entry for IGEM:Virginia/2012/Notebook/Genetically_engineered_bacteriophage_for_diagnosis_of_whooping_cough) |
(fix raw html notebook nav) |
||
(One intermediate revision by one other user not shown) | |||
Line 1: | Line 1: | ||
{|{{table}} width="800" | {|{{table}} width="800" | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week 11</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
== | ==(8/5 – 8/11)== | ||
We prepped the Biobrick plasmid p581c3 | |||
<br> | |||
We got results for miniprep of the plasmid backbone. Based on the nanodrop results, | |||
the procedure did not work. We tried growing cultures in liquid. | |||
<br> | |||
The cultures grew up in liquid but not as well as on plates. We made glycerol stocks | |||
and did plasmid prep followed by detection PCR to confirm presence of inserts. | |||
<br> | |||
We found no plaques on the T7 plate so we were unsure if T7 got in to the cell. | |||
<br> | |||
Did research on the protocol for refolding hCG-beta protein. SDS-PAGE suggested | |||
that there was no expression of the desired fragments. | |||
<br> | |||
Carried out T7 phage amplification. | |||
<br> | |||
Ordered antibodies to use in western blot | |||
<br> | |||
Planned out how to assemble promotor + RBS + hCG-beta Biobrick + terminator on | |||
the biobrick-designated plasmid | |||
Latest revision as of 22:05, 26 September 2017
Week 11 | Main project page Previous entry Next entry |
(8/5 – 8/11)We prepped the Biobrick plasmid p581c3
|