IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/08/26

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(Autocreate 2012/08/26 Entry for IGEM:Virginia/2012/Notebook/Genetically_engineered_bacteriophage_for_diagnosis_of_whooping_cough)
Current revision (18:35, 3 October 2012) (view source)
 
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;">Week of 8/26/12</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
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==Week of 8/26/12==
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* Insert your content here.
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• Transformed BBa_K331022 (which will be used for the pSB1C3 backbone), BBa_K091110 (which will be used for expressing genes under the control of the lacI promoter), and the SynCG plasmid into DH5α and grew them overnight on plates. The BBa_K331022 and SynCG plasmids grew on the plates.  These were then grown up in a liquid culture and plasmid prepped, resulting in 43.7 ng/µL
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• Digested bbp9 (E+S), bbp11 (E+S), promoter+RBS (E+S), BBa_K133022 (E+S), native hCG (E+S), SynCG plasmid (XbaI), and T7 genome (BclI).  Induced culture of DH5α of full hCG in vector with IPTG.  The bbp9, bbp11, K331022, and WT hCG produced suitable amounts after gel extraction.  However, the promoter+RBS needs to be redone and purified with ethanol precipitation along with the SynCG and the T7 genome.
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Week of 8/26/12

• Transformed BBa_K331022 (which will be used for the pSB1C3 backbone), BBa_K091110 (which will be used for expressing genes under the control of the lacI promoter), and the SynCG plasmid into DH5α and grew them overnight on plates. The BBa_K331022 and SynCG plasmids grew on the plates. These were then grown up in a liquid culture and plasmid prepped, resulting in 43.7 ng/µL
• Digested bbp9 (E+S), bbp11 (E+S), promoter+RBS (E+S), BBa_K133022 (E+S), native hCG (E+S), SynCG plasmid (XbaI), and T7 genome (BclI). Induced culture of DH5α of full hCG in vector with IPTG. The bbp9, bbp11, K331022, and WT hCG produced suitable amounts after gel extraction. However, the promoter+RBS needs to be redone and purified with ethanol precipitation along with the SynCG and the T7 genome.




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