IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/09/23
Week of 9/23/12 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Week of 9/23/12Redid backbone (with both E+S and E+P) but CIAPing the vector prior to ligation
in order to decrease the chance of the empty vector ligating to itself and creating
false positive colonies, producing acceptable concentrations of 90.4 and 71.0 ng/μL,
respectively. Colonies for the bbp9, hCG, SynCG+promotor, SynCG+promotor+RBS,,
plasmid (E+S), and plasmid (E+P) all are acceptable in the double digits. The SynCG
colony is still in the thousands. Inoculated colonies, performed plasmid prep, and
used PCR to confirm presence of inserts. hCG, SynCG, and P+RBS+SynCG all have
exceptionally large yields over 5000 ng/μL.
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