IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/09/30

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(Autocreate 2012/09/30 Entry for IGEM:Virginia/2012/Notebook/Genetically_engineered_bacteriophage_for_diagnosis_of_whooping_cough)
Current revision (20:10, 3 October 2012) (view source)
 
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;">Week of 9/30/12</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
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==Week of 9/30/12==
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* Insert your content here.
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Gel of the 12 PCR products showed no sign of the vector.
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<br>
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Repeated gel with P+RBS+SynCG plasmid #2 and plasmid #5, along with the 5 plasmid
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of P+RBS+SynCG from last week.
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<br>
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Performed pregnancy test experiments with SynCG biobricks in DH5α with two trials:
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one with only the #2 culture (the culture which the biobrick was extracted from) and one
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from the combined culture. These cultures were separated for sonication. Based off of
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previous results, no DTT will be added, only 8M Urea and no denaturing solution.

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Week of 9/30/12

Gel of the 12 PCR products showed no sign of the vector.
Repeated gel with P+RBS+SynCG plasmid #2 and plasmid #5, along with the 5 plasmid of P+RBS+SynCG from last week.
Performed pregnancy test experiments with SynCG biobricks in DH5α with two trials: one with only the #2 culture (the culture which the biobrick was extracted from) and one from the combined culture. These cultures were separated for sonication. Based off of previous results, no DTT will be added, only 8M Urea and no denaturing solution.



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