IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/09/30: Difference between revisions
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(Autocreate 2012/09/30 Entry for IGEM:Virginia/2012/Notebook/Genetically_engineered_bacteriophage_for_diagnosis_of_whooping_cough) |
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;">Week of 9/30/12</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==Week of 9/30/12== | ||
Gel of the 12 PCR products showed no sign of the vector. | |||
<br> | |||
Repeated gel with P+RBS+SynCG plasmid #2 and plasmid #5, along with the 5 plasmid | |||
of P+RBS+SynCG from last week. | |||
<br> | |||
Performed pregnancy test experiments with SynCG biobricks in DH5α with two trials: | |||
one with only the #2 culture (the culture which the biobrick was extracted from) and one | |||
from the combined culture. These cultures were separated for sonication. Based off of | |||
previous results, no DTT will be added, only 8M Urea and no denaturing solution. | |||
Latest revision as of 22:05, 26 September 2017
Week of 9/30/12 | Main project page Previous entry Next entry |
Week of 9/30/12Gel of the 12 PCR products showed no sign of the vector.
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