IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/10/03: Difference between revisions
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Crunch Time</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==October 3rd== | ||
Overnight pregnancy test results (~17 hours after the bacteria was added): | |||
<br> | |||
Positive: | |||
<br> | |||
Sonication, combination of all cultures, no DDT | |||
<br> | |||
T7 lysis, combination of all cultures, no DDT | |||
<br> | |||
Negative: | |||
<br> | |||
Son., culture #2 only, no DTT | |||
<br> | |||
T7, #2, no DTT | |||
<br> | |||
Son., comb, no denaturing solution added | |||
<br> | |||
T7, comb, no den | |||
<br> | |||
Son., #2, no den | |||
<br> | |||
T7, #2, no den | |||
<br> | |||
The negative tests showed one clear control band and a light background color which extended 3/4 of the way across the window. The background color was slightly darker in the area where the positive band would normally appear. | |||
<br> | |||
The Western blot showed bands at the appropriate molecular weight for hCG in all 4 lanes of promotor + RBS + synCG | |||
Latest revision as of 22:05, 26 September 2017
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October 3rdOvernight pregnancy test results (~17 hours after the bacteria was added):
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