IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/10/23

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(Autocreate 2012/10/23 Entry for IGEM:Virginia/2012/Notebook/Genetically_engineered_bacteriophage_for_diagnosis_of_whooping_cough)
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week of 10/23/12</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
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• The SynCG BioBrick was isolated again.
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* Insert your content here.
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• Due to the odd results of the last clearings, a 5 mL culture of BL21 was inoculated with material from the T7 and synCG plate.
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• Viral genome isolation was performed again with cell culture samples from each of the following: IDT (positive control); WildType T7 (negative control); T7 halves treated with CIAP; SynCG treated with CIAP; RBS and SynCG treated with CIAP; and RBS and SynCG not treated with CIAP.  However, upon performing PCR and gelling the products, no results formed.
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Revision as of 16:41, 26 October 2012

Week of 10/23/12 Main project page
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• The SynCG BioBrick was isolated again.
• Due to the odd results of the last clearings, a 5 mL culture of BL21 was inoculated with material from the T7 and synCG plate.
• Viral genome isolation was performed again with cell culture samples from each of the following: IDT (positive control); WildType T7 (negative control); T7 halves treated with CIAP; SynCG treated with CIAP; RBS and SynCG treated with CIAP; and RBS and SynCG not treated with CIAP. However, upon performing PCR and gelling the products, no results formed.




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