IGEM:Virginia 2012/Protocols: Difference between revisions
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==Solution & Plate Preparations== | ==Solution & Plate Preparations== | ||
* [[/Bordet Gengou (BG) Agar Plate Preparation|Bordet Gengou (BG) Agar Plate Preparation]] | * [[/Bordet Gengou (BG) Agar Plate Preparation|Bordet Gengou (BG) Agar Plate Preparation]] for culturing ''B. pertussis''. | ||
* [[/LB Broth or Agar Preparation|LB Broth or Agar Preparation]] | * [[/LB Broth or Agar Preparation|LB Broth or Agar Preparation]] for culturing ''B. bronchiseptica''. | ||
* [[/PEG/NaCl Preparation|PEG/NaCl Preparation]] | * [[/PEG/NaCl Preparation|PEG/NaCl Preparation]] | ||
* [[/SM Buffer Preparation|SM Buffer Preparation]] | * [[/SM Buffer Preparation|SM Buffer Preparation]] | ||
* [[/SSM Media Preparation|SSM Media Preparation]] | * [[/SSM Media Preparation|SSM Media Preparation]] | ||
Revision as of 12:20, 31 May 2012
Here is a list of the protocols the 2012 Virginia iGEM team will be following in order to carry out our research project.
Protocols
- Phage Amplification
- Plaque Assay -- to quantify the amount of bacteriophages in our samples.
- Phage Isolation
- Phage Replication Rate Assay
- Preparing a Pertussis Culture
Solution & Plate Preparations
- Bordet Gengou (BG) Agar Plate Preparation for culturing B. pertussis.
- LB Broth or Agar Preparation for culturing B. bronchiseptica.
- PEG/NaCl Preparation
- SM Buffer Preparation
- SSM Media Preparation
