IGEM:Virginia 2012/Protocols: Difference between revisions
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* [[/SM Buffer Preparation|SM Buffer Preparation]] | * [[/SM Buffer Preparation|SM Buffer Preparation]] | ||
* [[/SSM Media Preparation|SSM Media Preparation]] | * [[/SSM Media Preparation|SSM Media Preparation]] | ||
* [http://en.wikipedia.org/wiki/TE_buffer TE Buffer] | |||
Revision as of 07:23, 4 June 2012
Here is a list of the protocols the 2012 Virginia iGEM team will be following in order to carry out our research project.
Protocols
- Phage Amplification
- Plaque Assay -- to quantify the amount of bacteriophages in our samples.
- Phage Isolation
- Phage Replication Rate Assay
- Preparing a Pertussis Culture
- Isolating the Phage Genome
Solution & Plate Preparations
- Bordet Gengou (BG) Agar Plate Preparation for culturing B. pertussis.
- LB Broth or Agar Preparation for culturing B. bronchiseptica.
- PEG/NaCl Preparation
- SM Buffer Preparation
- SSM Media Preparation
- TE Buffer
