IGEM:Virginia 2012/Protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
| Line 8: | Line 8: | ||
* [[/Preparing a Pertussis Culture|Preparing a Pertussis Culture]] | * [[/Preparing a Pertussis Culture|Preparing a Pertussis Culture]] | ||
* [[/Isolating the Phage Genome|Isolating the Phage Genome]] | * [[/Isolating the Phage Genome|Isolating the Phage Genome]] | ||
* [[/CaCl2 Competent Stock Cells | CaCl2 Competent Stock Cells]] | |||
==Solution & Plate Preparations== | ==Solution & Plate Preparations== | ||
Revision as of 07:25, 4 June 2012
Here is a list of the protocols the 2012 Virginia iGEM team will be following in order to carry out our research project.
Protocols
- Phage Amplification
- Plaque Assay -- to quantify the amount of bacteriophages in our samples.
- Phage Isolation
- Phage Replication Rate Assay
- Preparing a Pertussis Culture
- Isolating the Phage Genome
- CaCl2 Competent Stock Cells
Solution & Plate Preparations
- Bordet Gengou (BG) Agar Plate Preparation for culturing B. pertussis.
- LB Broth or Agar Preparation for culturing B. bronchiseptica.
- PEG/NaCl Preparation
- SM Buffer Preparation
- SSM Media Preparation
- TE Buffer
