IGEM:Virginia 2012/Protocols

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(Protocols)
Current revision (17:42, 2 October 2012) (view source)
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==Protocols==
==Protocols==
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* [[/Plaque Assay|Plaque Assay]] -- to quantify the amount of bacteriophages in our samples.
 
* [[/Phage Amplification|Phage Amplification]]
* [[/Phage Amplification|Phage Amplification]]
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* [[/Plaque Assay|Plaque Assay]] -- to quantify the amount of bacteriophage in a sample.
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* [[/Phage Isolation|Phage Isolation]]
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* [[/Phage Replication Rate Assay|Phage Replication Rate Assay]]
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* [[/Preparing a Pertussis Culture|Preparing a Pertussis Culture]]
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* [[/Isolating the Phage Genome|Isolating the Phage Genome]]
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* [[/CaCl2 Competent Stock Cells | CaCl2 Competent Stock Cells]]
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* [http://tools.invitrogen.com/content/sfs/manuals/subcloningefficiencydh5alpha_man.pdf Chemical transformation]
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* [[/Using the NanoDrop | Using the NanoDrop]]
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==Solution & Plate Preparations==
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* [[/Bordet Gengou (BG) Agar Plate Preparation|Bordet Gengou (BG) Agar Plate Preparation]] for culturing ''B. pertussis''.
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* [[/LB Broth or Agar Preparation|LB Broth or Agar Preparation]] for culturing ''B. bronchiseptica''.
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* [[/PEG/NaCl Preparation|PEG/NaCl Preparation]]
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* [[/SM Buffer Preparation|SM Buffer Preparation]]
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* [[/SSM Media Preparation|SSM Media Preparation]]
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* [http://en.wikipedia.org/wiki/TE_buffer TE Buffer]

Current revision

Here is a list of the protocols the 2012 Virginia iGEM team will be following in order to carry out our research project.

Protocols

Solution & Plate Preparations

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