IGEM:Virginia 2012/Protocols
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==Protocols== | ==Protocols== | ||
| - | |||
* [[/Phage Amplification|Phage Amplification]] | * [[/Phage Amplification|Phage Amplification]] | ||
| + | * [[/Plaque Assay|Plaque Assay]] -- to quantify the amount of bacteriophage in a sample. | ||
* [[/Phage Isolation|Phage Isolation]] | * [[/Phage Isolation|Phage Isolation]] | ||
| + | * [[/Phage Replication Rate Assay|Phage Replication Rate Assay]] | ||
| + | * [[/Preparing a Pertussis Culture|Preparing a Pertussis Culture]] | ||
| + | * [[/Isolating the Phage Genome|Isolating the Phage Genome]] | ||
| + | * [[/CaCl2 Competent Stock Cells | CaCl2 Competent Stock Cells]] | ||
| + | * [http://tools.invitrogen.com/content/sfs/manuals/subcloningefficiencydh5alpha_man.pdf Chemical transformation] | ||
| + | * [[/Using the NanoDrop | Using the NanoDrop]] | ||
| + | |||
| + | ==Solution & Plate Preparations== | ||
| + | * [[/Bordet Gengou (BG) Agar Plate Preparation|Bordet Gengou (BG) Agar Plate Preparation]] for culturing ''B. pertussis''. | ||
| + | * [[/LB Broth or Agar Preparation|LB Broth or Agar Preparation]] for culturing ''B. bronchiseptica''. | ||
| + | * [[/PEG/NaCl Preparation|PEG/NaCl Preparation]] | ||
| + | * [[/SM Buffer Preparation|SM Buffer Preparation]] | ||
| + | * [[/SSM Media Preparation|SSM Media Preparation]] | ||
| + | * [http://en.wikipedia.org/wiki/TE_buffer TE Buffer] | ||
Current revision
Here is a list of the protocols the 2012 Virginia iGEM team will be following in order to carry out our research project.
Protocols
- Phage Amplification
- Plaque Assay -- to quantify the amount of bacteriophage in a sample.
- Phage Isolation
- Phage Replication Rate Assay
- Preparing a Pertussis Culture
- Isolating the Phage Genome
- CaCl2 Competent Stock Cells
- Chemical transformation
- Using the NanoDrop
Solution & Plate Preparations
- Bordet Gengou (BG) Agar Plate Preparation for culturing B. pertussis.
- LB Broth or Agar Preparation for culturing B. bronchiseptica.
- PEG/NaCl Preparation
- SM Buffer Preparation
- SSM Media Preparation
- TE Buffer
