IGEM:Virginia 2012/Protocols: Difference between revisions

Latest revision as of 15:42, 2 October 2012

Here is a list of the protocols the 2012 Virginia iGEM team will be following in order to carry out our research project.

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 ==Protocols==
-* [[/Plaque Assay|Plaque Assay]] -- to quantify the amount of bacteriophages in our samples.
 * [[/Phage Amplification|Phage Amplification]]
+* [[/Plaque Assay|Plaque Assay]] -- to quantify the amount of bacteriophage in a sample.
 * [[/Phage Isolation|Phage Isolation]]
 * [[/Phage Replication Rate Assay|Phage Replication Rate Assay]]
-* [[/Preparing Plates and Liquid Media|Preparing Plates and Liquid Media]]
+* [[/Preparing a Pertussis Culture|Preparing a Pertussis Culture]]
-* [[/Solutions|Solutions]]
+* [[/Isolating the Phage Genome|Isolating the Phage Genome]]
+* [[/CaCl2 Competent Stock Cells | CaCl2 Competent Stock Cells]]
+* [http://tools.invitrogen.com/content/sfs/manuals/subcloningefficiencydh5alpha_man.pdf Chemical transformation]
+* [[/Using the NanoDrop | Using the NanoDrop]]
+==Solution & Plate Preparations==
+* [[/Bordet Gengou (BG) Agar Plate Preparation|Bordet Gengou (BG) Agar Plate Preparation]] for culturing ''B. pertussis''.
+* [[/LB Broth or Agar Preparation|LB Broth or Agar Preparation]] for culturing ''B. bronchiseptica''.
+* [[/PEG/NaCl Preparation|PEG/NaCl Preparation]]
+* [[/SM Buffer Preparation|SM Buffer Preparation]]
+* [[/SSM Media Preparation|SSM Media Preparation]]
+* [http://en.wikipedia.org/wiki/TE_buffer TE Buffer]