IGEM:Virginia 2012/Protocols
From OpenWetWare
< IGEM:Virginia 2012(Difference between revisions)
(→Protocols) |
Current revision (18:42, 2 October 2012) (view source) m |
||
| (4 intermediate revisions not shown.) | |||
| Line 3: | Line 3: | ||
==Protocols== | ==Protocols== | ||
* [[/Phage Amplification|Phage Amplification]] | * [[/Phage Amplification|Phage Amplification]] | ||
| - | * [[/Plaque Assay|Plaque Assay]] -- to quantify the amount of | + | * [[/Plaque Assay|Plaque Assay]] -- to quantify the amount of bacteriophage in a sample. |
* [[/Phage Isolation|Phage Isolation]] | * [[/Phage Isolation|Phage Isolation]] | ||
* [[/Phage Replication Rate Assay|Phage Replication Rate Assay]] | * [[/Phage Replication Rate Assay|Phage Replication Rate Assay]] | ||
| Line 9: | Line 9: | ||
* [[/Isolating the Phage Genome|Isolating the Phage Genome]] | * [[/Isolating the Phage Genome|Isolating the Phage Genome]] | ||
* [[/CaCl2 Competent Stock Cells | CaCl2 Competent Stock Cells]] | * [[/CaCl2 Competent Stock Cells | CaCl2 Competent Stock Cells]] | ||
| - | * [ | + | * [http://tools.invitrogen.com/content/sfs/manuals/subcloningefficiencydh5alpha_man.pdf Chemical transformation] |
| - | * [[Using the NanoDrop | Using the NanoDrop]] | + | * [[/Using the NanoDrop | Using the NanoDrop]] |
==Solution & Plate Preparations== | ==Solution & Plate Preparations== | ||
Current revision
Here is a list of the protocols the 2012 Virginia iGEM team will be following in order to carry out our research project.
Protocols
- Phage Amplification
- Plaque Assay -- to quantify the amount of bacteriophage in a sample.
- Phage Isolation
- Phage Replication Rate Assay
- Preparing a Pertussis Culture
- Isolating the Phage Genome
- CaCl2 Competent Stock Cells
- Chemical transformation
- Using the NanoDrop
Solution & Plate Preparations
- Bordet Gengou (BG) Agar Plate Preparation for culturing B. pertussis.
- LB Broth or Agar Preparation for culturing B. bronchiseptica.
- PEG/NaCl Preparation
- SM Buffer Preparation
- SSM Media Preparation
- TE Buffer
