IGEM:Virginia 2012/Protocols
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< IGEM:Virginia 2012(Difference between revisions)
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==Protocols== | ==Protocols== | ||
* [[/Phage Amplification|Phage Amplification]] | * [[/Phage Amplification|Phage Amplification]] | ||
| - | * [[/Plaque Assay|Plaque Assay]] -- to quantify the amount of bacteriophage in a | + | * [[/Plaque Assay|Plaque Assay]] -- to quantify the amount of bacteriophage in a sample. |
* [[/Phage Isolation|Phage Isolation]] | * [[/Phage Isolation|Phage Isolation]] | ||
* [[/Phage Replication Rate Assay|Phage Replication Rate Assay]] | * [[/Phage Replication Rate Assay|Phage Replication Rate Assay]] | ||
Current revision
Here is a list of the protocols the 2012 Virginia iGEM team will be following in order to carry out our research project.
Protocols
- Phage Amplification
- Plaque Assay -- to quantify the amount of bacteriophage in a sample.
- Phage Isolation
- Phage Replication Rate Assay
- Preparing a Pertussis Culture
- Isolating the Phage Genome
- CaCl2 Competent Stock Cells
- Chemical transformation
- Using the NanoDrop
Solution & Plate Preparations
- Bordet Gengou (BG) Agar Plate Preparation for culturing B. pertussis.
- LB Broth or Agar Preparation for culturing B. bronchiseptica.
- PEG/NaCl Preparation
- SM Buffer Preparation
- SSM Media Preparation
- TE Buffer
