IGEM:Virginia 2012/Protocols - Revision history

Joseph J. Muldoon at 22:42, 2 October 2012

Joseph J. Muldoon — Tue, 02 Oct 2012 22:42:35 GMT

←Older revision		Revision as of 22:42, 2 October 2012
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	==Protocols==		==Protocols==
	* [[/Phage Amplification\|Phage Amplification]]		* [[/Phage Amplification\|Phage Amplification]]
-	* [[/Plaque Assay\|Plaque Assay]] -- to quantify the amount of bacteriophage in a ~~samples~~.	+	* [[/Plaque Assay\|Plaque Assay]] -- to quantify the amount of bacteriophage in a sample.
	* [[/Phage Isolation\|Phage Isolation]]		* [[/Phage Isolation\|Phage Isolation]]
	* [[/Phage Replication Rate Assay\|Phage Replication Rate Assay]]		* [[/Phage Replication Rate Assay\|Phage Replication Rate Assay]]

Joseph J. Muldoon — Tue, 02 Oct 2012 22:42:19 GMT

←Older revision		Revision as of 22:42, 2 October 2012
Line 3:		Line 3:
	==Protocols==		==Protocols==
	* [[/Phage Amplification\|Phage Amplification]]		* [[/Phage Amplification\|Phage Amplification]]
-	* [[/Plaque Assay\|Plaque Assay]] -- to quantify the amount of ~~bacteriophages~~ in ~~our~~ samples.	+	* [[/Plaque Assay\|Plaque Assay]] -- to quantify the amount of bacteriophage in a samples.
	* [[/Phage Isolation\|Phage Isolation]]		* [[/Phage Isolation\|Phage Isolation]]
	* [[/Phage Replication Rate Assay\|Phage Replication Rate Assay]]		* [[/Phage Replication Rate Assay\|Phage Replication Rate Assay]]

Joseph J. Muldoon — Mon, 04 Jun 2012 20:09:57 GMT

←Older revision		Revision as of 20:09, 4 June 2012
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	* [[/Isolating the Phage Genome\|Isolating the Phage Genome]]		* [[/Isolating the Phage Genome\|Isolating the Phage Genome]]
	* [[/CaCl2 Competent Stock Cells \| CaCl2 Competent Stock Cells]]		* [[/CaCl2 Competent Stock Cells \| CaCl2 Competent Stock Cells]]
		+	* [http://tools.invitrogen.com/content/sfs/manuals/subcloningefficiencydh5alpha_man.pdf Chemical transformation]
	* [[/Using the NanoDrop \| Using the NanoDrop]]		* [[/Using the NanoDrop \| Using the NanoDrop]]

Jacqueline K. Grimm — Mon, 04 Jun 2012 18:22:26 GMT

←Older revision		Revision as of 18:22, 4 June 2012
Line 9:		Line 9:
	* [[/Isolating the Phage Genome\|Isolating the Phage Genome]]		* [[/Isolating the Phage Genome\|Isolating the Phage Genome]]
	* [[/CaCl2 Competent Stock Cells \| CaCl2 Competent Stock Cells]]		* [[/CaCl2 Competent Stock Cells \| CaCl2 Competent Stock Cells]]
-	* [[/Passaging Pertussis \| Passaging Pertussis]]
	* [[/Using the NanoDrop \| Using the NanoDrop]]		* [[/Using the NanoDrop \| Using the NanoDrop]]

Alex Zorychta — Mon, 04 Jun 2012 16:37:03 GMT

←Older revision		Revision as of 16:37, 4 June 2012
Line 10:		Line 10:
	* [[/CaCl2 Competent Stock Cells \| CaCl2 Competent Stock Cells]]		* [[/CaCl2 Competent Stock Cells \| CaCl2 Competent Stock Cells]]
	* [[/Passaging Pertussis \| Passaging Pertussis]]		* [[/Passaging Pertussis \| Passaging Pertussis]]
-	* [[Using the NanoDrop \| Using the NanoDrop]]	+	* [[/Using the NanoDrop \| Using the NanoDrop]]

	==Solution & Plate Preparations==		==Solution & Plate Preparations==

Alex Zorychta — Mon, 04 Jun 2012 16:28:30 GMT

Protocols

←Older revision		Revision as of 16:28, 4 June 2012
Line 10:		Line 10:
	* [[/CaCl2 Competent Stock Cells \| CaCl2 Competent Stock Cells]]		* [[/CaCl2 Competent Stock Cells \| CaCl2 Competent Stock Cells]]
	* [[/Passaging Pertussis \| Passaging Pertussis]]		* [[/Passaging Pertussis \| Passaging Pertussis]]
		+	* [[Using the NanoDrop \| Using the NanoDrop]]

	==Solution & Plate Preparations==		==Solution & Plate Preparations==

Alex Zorychta — Mon, 04 Jun 2012 16:14:13 GMT

Protocols

←Older revision		Revision as of 16:14, 4 June 2012
Line 9:		Line 9:
	* [[/Isolating the Phage Genome\|Isolating the Phage Genome]]		* [[/Isolating the Phage Genome\|Isolating the Phage Genome]]
	* [[/CaCl2 Competent Stock Cells \| CaCl2 Competent Stock Cells]]		* [[/CaCl2 Competent Stock Cells \| CaCl2 Competent Stock Cells]]
		+	* [[/Passaging Pertussis \| Passaging Pertussis]]

	==Solution & Plate Preparations==		==Solution & Plate Preparations==

Alex Zorychta — Mon, 04 Jun 2012 14:25:25 GMT

Protocols

←Older revision		Revision as of 14:25, 4 June 2012
Line 8:		Line 8:
	* [[/Preparing a Pertussis Culture\|Preparing a Pertussis Culture]]		* [[/Preparing a Pertussis Culture\|Preparing a Pertussis Culture]]
	* [[/Isolating the Phage Genome\|Isolating the Phage Genome]]		* [[/Isolating the Phage Genome\|Isolating the Phage Genome]]
		+	* [[/CaCl2 Competent Stock Cells \| CaCl2 Competent Stock Cells]]

	==Solution & Plate Preparations==		==Solution & Plate Preparations==

Alex Zorychta — Mon, 04 Jun 2012 14:23:33 GMT

Solution & Plate Preparations

←Older revision		Revision as of 14:23, 4 June 2012
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	* [[/SM Buffer Preparation\|SM Buffer Preparation]]		* [[/SM Buffer Preparation\|SM Buffer Preparation]]
	* [[/SSM Media Preparation\|SSM Media Preparation]]		* [[/SSM Media Preparation\|SSM Media Preparation]]
		+	* [http://en.wikipedia.org/wiki/TE_buffer TE Buffer]

Alex Zorychta — Fri, 01 Jun 2012 16:22:51 GMT

Protocols

←Older revision		Revision as of 16:22, 1 June 2012
Line 7:		Line 7:
	* [[/Phage Replication Rate Assay\|Phage Replication Rate Assay]]		* [[/Phage Replication Rate Assay\|Phage Replication Rate Assay]]
	* [[/Preparing a Pertussis Culture\|Preparing a Pertussis Culture]]		* [[/Preparing a Pertussis Culture\|Preparing a Pertussis Culture]]
		+	* [[/Isolating the Phage Genome\|Isolating the Phage Genome]]

	==Solution & Plate Preparations==		==Solution & Plate Preparations==

←Older revision		Revision as of 22:42, 2 October 2012
Line 3:		Line 3:
	==Protocols==		==Protocols==
	* [[/Phage Amplification\|Phage Amplification]]		* [[/Phage Amplification\|Phage Amplification]]
-	* [[/Plaque Assay\|Plaque Assay]] -- to quantify the amount of bacteriophage in a ~~samples~~.	+	* [[/Plaque Assay\|Plaque Assay]] -- to quantify the amount of bacteriophage in a sample.
	* [[/Phage Isolation\|Phage Isolation]]		* [[/Phage Isolation\|Phage Isolation]]
	* [[/Phage Replication Rate Assay\|Phage Replication Rate Assay]]		* [[/Phage Replication Rate Assay\|Phage Replication Rate Assay]]

←Older revision		Revision as of 22:42, 2 October 2012
Line 3:		Line 3:
	==Protocols==		==Protocols==
	* [[/Phage Amplification\|Phage Amplification]]		* [[/Phage Amplification\|Phage Amplification]]
-	* [[/Plaque Assay\|Plaque Assay]] -- to quantify the amount of ~~bacteriophages~~ in ~~our~~ samples.	+	* [[/Plaque Assay\|Plaque Assay]] -- to quantify the amount of bacteriophage in a samples.
	* [[/Phage Isolation\|Phage Isolation]]		* [[/Phage Isolation\|Phage Isolation]]
	* [[/Phage Replication Rate Assay\|Phage Replication Rate Assay]]		* [[/Phage Replication Rate Assay\|Phage Replication Rate Assay]]

←Older revision		Revision as of 20:09, 4 June 2012
Line 9:		Line 9:
	* [[/Isolating the Phage Genome\|Isolating the Phage Genome]]		* [[/Isolating the Phage Genome\|Isolating the Phage Genome]]
	* [[/CaCl2 Competent Stock Cells \| CaCl2 Competent Stock Cells]]		* [[/CaCl2 Competent Stock Cells \| CaCl2 Competent Stock Cells]]
		+	* [http://tools.invitrogen.com/content/sfs/manuals/subcloningefficiencydh5alpha_man.pdf Chemical transformation]
	* [[/Using the NanoDrop \| Using the NanoDrop]]		* [[/Using the NanoDrop \| Using the NanoDrop]]

←Older revision		Revision as of 18:22, 4 June 2012
Line 9:		Line 9:
	* [[/Isolating the Phage Genome\|Isolating the Phage Genome]]		* [[/Isolating the Phage Genome\|Isolating the Phage Genome]]
	* [[/CaCl2 Competent Stock Cells \| CaCl2 Competent Stock Cells]]		* [[/CaCl2 Competent Stock Cells \| CaCl2 Competent Stock Cells]]
-	* [[/Passaging Pertussis \| Passaging Pertussis]]
	* [[/Using the NanoDrop \| Using the NanoDrop]]		* [[/Using the NanoDrop \| Using the NanoDrop]]