IGEM:Virginia 2012/Protocols/PEG/NaCl Preparation: Difference between revisions
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# Fill a 1 L bottle with 400 mL of milliQ H2O and put in a stir bar. Use the magnetic mixer to stir while you add the PEG and NaCl. | # Fill a 1 L bottle with 400 mL of milliQ H2O and put in a stir bar. Use the magnetic mixer to stir while you add the PEG and NaCl. | ||
# Measure out 80g of PEG-8000 and add it slowly (it might be easier to add | # Measure out 80g of PEG-8000 and add it slowly (it might be easier to add it in smaller increments). | ||
# Measure 58.4g NaCl and add it slowly. | # Measure 58.4g NaCl and add it slowly. | ||
# Autoclave (large liquid). | |||
# Stir it on the magnetic mixer again. | |||
==Notes== | ==Notes== | ||
Latest revision as of 11:09, 30 May 2012
Materials
- 20% PEG-8000
- 2.5 M NaCl
- milliQ H2O
- 1 L bottle
- Stir bar
Procedure
- Fill a 1 L bottle with 400 mL of milliQ H2O and put in a stir bar. Use the magnetic mixer to stir while you add the PEG and NaCl.
- Measure out 80g of PEG-8000 and add it slowly (it might be easier to add it in smaller increments).
- Measure 58.4g NaCl and add it slowly.
- Autoclave (large liquid).
- Stir it on the magnetic mixer again.
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
References
Contact
- Who has experience with this protocol?
or instead, discuss this protocol.
