IGEM:Virginia 2012/Protocols/PEG/NaCl Preparation

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Current revision (14:09, 30 May 2012) (view source)
 
Line 12: Line 12:
# Measure out 80g of PEG-8000 and add it slowly (it might be easier to add it in smaller increments).
# Measure out 80g of PEG-8000 and add it slowly (it might be easier to add it in smaller increments).
# Measure 58.4g NaCl and add it slowly.
# Measure 58.4g NaCl and add it slowly.
 +
# Autoclave (large liquid).
 +
# Stir it on the magnetic mixer again.
==Notes==
==Notes==

Current revision

Contents

Materials

  • 20% PEG-8000
  • 2.5 M NaCl
  • milliQ H2O
  • 1 L bottle
  • Stir bar

Procedure

  1. Fill a 1 L bottle with 400 mL of milliQ H2O and put in a stir bar. Use the magnetic mixer to stir while you add the PEG and NaCl.
  2. Measure out 80g of PEG-8000 and add it slowly (it might be easier to add it in smaller increments).
  3. Measure 58.4g NaCl and add it slowly.
  4. Autoclave (large liquid).
  5. Stir it on the magnetic mixer again.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!


References

Contact

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or instead, discuss this protocol.

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